Upright multiphoton microscope for rapid 4D image acquisition in live specimens. Ideal for imaging diverse samples from tissue slices to whole organisms. Also has a CSU-X1 spinning disk confocal.
Capabilities
- Multiphoton
- FRAP
- Spinning Disk Confocal
- Live specimens
Objectives:
- Zeiss W Plan-Apochromat water dipping objective, 20x/1.0NA, 2.4mm working distance
Equipment Details:
- Zeiss AxioExaminer Stand
- Luigs & Neumann Shifting Table
- Luigs & Neumann Temperature controller VII
- PI PZT objective Piezo
- Conoptics Pockel Cell
- CSU-X1 FW spinning disk with filter wheel
- Photometrics Prime 95B (Spinning Disk)
- X-Cite 200DC epi lamp
- GaAsP PMTs
- Dual galvanometer scanner
- Resonant scanner
Light Source:
- Coherent Chameleon Ultra
- 3i Laser Stack with 488nm and 561nm lasers (Spinning Disk)
- 3i Laser Stack with 594nm laser (Photostim)
Emission Filters:
- 612/69 (PMT 1)
- 525/40 (PMT 2)
Emission Filters for Widefield Epifluorscence:
- GFP cube
- Texas Red cube
Emission Filters for Spinning Disk Confocal:
- 617/73
- 525/30
Location:
Weill Neurosciences Building
Room:
Weill 551E
Core:
CALM (CAMM)
Technique:
Confocal
FRAP/Photoactivation
Live Cell
Multi-photon
Upright microscope
Staff contact:
Kari Herrington, SoYeon Kim
Special Access requirements:
Laser Safety
Training by Core Staff
Grants/Funding to be acknowledged:
Weston Havens Foundation and Kavli Institute for Fundamental Neuroscience