The Leica SPE5 is an inverted point-scanning microscope system with an AOBS tunable beam-splitter for great flexibility with fluorophore combinations. This microscope is equipped with an automated stage that can be used for automated imaging and an incubator (temperature only). The microscope can be used for 3-channel simultaneous acquisition, and live cell dynamics (FRAM, FRET, and GCaMP) at room temperature. Please note this microscope is not suited for imaging DAPI/dyes requiring a 405 excitation, low-light fluorescence imaging, High-speed fluorescence imaging, live cell imaging requiring CO₂, or samples thicker than 100um.

Capabilities

• Fixed and live-cell confocal imaging at room temperature
• Up to 3-channel simultaneous acquisition
• AOBS tunable beam-splitter for flexibility with fluorophore combinations
• Live-cell dynamics (FRAP, FRET, GCaMP)
• Multipositional acquisition and tiling multiple images for large sample areas 

Objectives:

• HCX PL FLUOTAR 10x/0.3
• HC PL FLUOTAR 20X/0.5
• HC PL APO (CORR) 40X/0.85
• HCX PL APO 63X/1.4 Oil

Light Source:

• Leica Laser lines 
• Metal halide illumination (for finding samples)

Excitation Wavelengths:

• 458nm
• 488nm
• 514nm
• 543nm
• 633nm