The Nikon N-SIM is a 4 color super resolution microscope that utilizes Structured Illumination to achieve resolutions of 120nm in XY and ~350nm in Z.
Capabilities
- Super-resolution imaging by structured illumination (up to 120 nm in XY, 350 nm in Z)
- 4-color fluorescence imaging
Objectives:
- Plan Apo 10x/0.45
- Plan Fluor ELWD 40x/0.65
- Plan Apo IR 60x/1.27 Water
- Apo TIRF SR 100x/1.49 Oil
Equipment Details:
- Nikon Ti Microscope (Inverted) with N-SIM
- SIM Gratings
- 3D EX V-G 100X/1.49 (for 100x objective, 405nm - 561nm)
- 3D EX V-R 100x/1.49 (for 100x objective, 405nm - 640nm)
- 3D EX V-R 60x/1.27 - TIRF 488 100x/1.49 (for 60x objective, 405nm - 640nm or 100x objective, 488nm TIRF)
- TIRF561 100x/1.49
- 3D 1layer 100x/1.49
- 3D 1layer 60x/1.27 - TIRF488 100x/1.49
- Nikon LUSU laser controller
- Nikon motorized stage
- Andor DU-897 EMCCD
Light Source:
- Nikon Laser Lanch
Excitation Wavelengths:
- 405nm
- 488nm
- 561nm
- 640nm
Emission Filters:
- SIM 405
- SIM 488
- SIM 561
- SIM 640
Location:
Genentech Hall
Core:
CALM (NIC)
Type:
Light
Technique:
Inverted microscope
Super-resolution
Staff contact:
Kari Herrington, SoYeon Kim
Special Access requirements:
Laser Safety
Training by Core Staff