3i VIVO Moveable Objective Microscope (MOM) with Phasor

3i Multiphoton Movable Objective Microscope with Phasor for holographic photostimulation.

Links

iLab Calendar

 FPbase

Core WEbPage

 

Capabilities

  • 2P Phasor for optogenetic stimulation
  • Open architechture with large space under the objective 
  • Dual galvanometer bidirectional scanning
    • Up to 6 frames/3000lines/2,500,00 samples per sec with max frame size of 4096x4096px

 

 

 

Objectives:

  • Zeiss Plan Apo 20x/1.0 NA W, 2.4mm WD

 

Equipment Details:

  • Sutter MOM
  • Dual galvanometer scanner
  • Resonant scanner
  • 3i Phasor holography
  • 2 GaAsP PMTs

Light Source:

  • Coherent Discovery Ti:Saph laser
  • Spectral-Physics FemtoTrain laser
  • X-Cite 110 LED (Widefield)

 

Emission Filters for PMTs:

  • 612/69 (PMT 1)
  • 525/40 (PMT 2)

Emission Filters for Widefield Epifluorscence:

  • GFP cube
  • Texas Red cube

 

Location: 
Weill Neurosciences Building
Room: 
Weill 551E
Core: 
CALM (CAMM)
Technique: 
FRAP/Photoactivation
Multi-photon
Upright microscope
Staff contact: 
Kari Herrington, Soyeon Kim
Special Access requirements: 
Laser Safety
Training by Core Staff
Grants/Funding to be acknowledged: 
Weston Havens Foundation and Kavli Institute for Fundamental Neuroscience