The 6D widefield microscope is an inverted widefield microscope equipped with cameras for 5-color fluorescent and color bright field imaging. This system can be used for imaging slides and multiwell plates utilizing a automated stage, Nikon Perfect Focus system, and Nikons High Content Analysis (HCA) module.
Capabilities
- Color brightfield imaging
- 5-color fluorescence imaging
- Brightfield, DIC, and Phase
- Automated plate scanning
- Automated image stitching
Objectives:
- Plan Apo 4x/0.2
- Plan Apo10x/0.45
- Plan Apo 20x/0.75
- Plan Apo 40x/0.95 Corr
- Plan Apo VC 60x/1.4 Oil
- Plan Apo VC 100x/1.4 Oil
Equipment Details:
- Nikon Ti Inverted Microscope
- Nikon DS-Qi2 monochrome camera
- DS-Ri2 color camera
- Sutter Lambda LS-2 xenon arc lamp
- Sutter Lambda 10-3 Filter changer
Light Source:
- Sutter Lambda LS-2 xenon arc lamp with cold mirror installed
Excitation Filters:
- 387/11x Semrock 5-band
- 485/20x Semrock 5-band
- 560/25x Semrock 5-band
- 650/13x Semrock 5-band
- 740/13x Semrock 5-band
- ET470/40x GFP/mCherry ET (89021 Ex2)
- ET572/35x GFP/mCherry ET (89021 Ex1)
- ET430/24x CFP/YFP (89002 Ex1)
- ET500/20x CFP/YFP (89002 Ex2)
Emission Filters:
- 440/40m Semrock 5-band
- 525/30m Semrock 5-band
- 607/36m Semrock 5-band
- 684/24m Semrock 5-band
- 809/81m Semrock 5-band
- ET525/50m GFP/mCherry ET (89021 Em2)
- ET632/60m GFP/mCherry ET (89021 Em1)
- ET470/24m CFP/YFP (89002 Em2)
- ET535/30m CFP/YFP (89000 Em1)
Location:
Genentech Hall
Room:
GH S252
Core:
CALM (NIC)
Type:
Light
Technique:
Brightfield, DIC, phase
HCA on Multi-well plates
Inverted microscope
Widefield epifluorescence
Staff contact:
Kari Herrington, SoYeon Kim
Special Access requirements:
Training by Core Staff