AZ100 Ultra Microscope Lightsheet

AZ100 Ultra microscope Lightsheet

The AZ100 ultra microscope that is equipped with a light sheet and set up for macro imaging of cleared tissue samples up to 2cm a side at cellular resolution. The system is capable of 4-color imaging and allows for imaging of tissues cleared with organic solvents.

 

Capabilities

  • Light Sheet imaging of cleared tissues up to 2 cm on a side
  • 4-color fluorescence imaging
  • 3D imaging
  • Automated image stitching

 

 


 

Objectives:

  • 1x / 0.1 AZ Plan Apo
  • 2x / 0.2 AZ Plan Apo
  • 5x /0.5 AZ Plan Apo

 

Equipment Details:

  • Nikon AZ-100 microscope
  • Vortran 4-line laser launch
  • Andor Zyla 5.5 camera
  • ASI motorized stage
  • Custom light sheet optics

Light Source:

  • Vortran Laser Launch

 

Excitation Wavelengths 

  • 405nm
  • 488nm
  • 561nm
  • 640nm

 

Emission Filters:

  • ET455/50m
  • ET535/36m
  • ET605/52m
  • ET705/72m

 

Location: 
Genentech Hall
Room: 
GH S252
Core: 
CALM (NIC)
Type: 
Light
Technique: 
Lightsheet
Upright microscope
Staff contact: 
Kari Herrington, SoYeon Kim
Special Access requirements: 
Laser Safety
Training by Core Staff