This inverted Nikon Ti2-E microscope is equipped with a CREST X-Light V2 large field of view spinning disk confocal, a C2-DUVB laser scanning confocal and an FRAP unit with a 405 nm Laser for photoactivation and photobleaching.  The Crest X-Light V2 spinning has the capability of a 25mm field of view and disk is equipped with a Prime 95 25MM sCMOS camera that has a high sensitivity (95% QE) and a Celesta Light system with 7 laser lines (405, 446, 477, 520, 546, 638, 749nm). The C2-DUVB is a 4 channel (405, 488, 561, 640nm) laser scanner with a GaAsP  detector unit that can be used for spectral imaging. The microscope utilizes the Nikon Perfect Focus System, an automated piezo stage to enable multi-position high throughput imaging. It is also equipped with an Oko cage incubator and  5% CO2 making it ideal for both fixed and live specimen imaging.

Capabilities

• 7  laser lines (405, 446, 477, 520, 546, 638, 749nm) for the spinning disk confocal
• 4 Laser lines (405, 488, 561, 647) for the laser scanner confocal
• Easily switch between spinning disk and laser scanner
• 3D confocal Imaging
• Gigapixel image stitching
• Automated Imaging
• Live cell time lapse imaging
• Photobleaching and photo activation
• Spectral Imaging

Objectives:

• CFI Plan Apo Lambda 10x /0.45
• CFI Plan Apo Lambda 20x/0.75
• CFI Plan Apo Lambda 40x/0.95 Corr
• CFI Plan Apo Lambda S 40x/1.25 Corr (Silicone)
• CFI Plan Apo Lambda 60x/1.4

Light Source:

• Lumencor Sola SE U-NIR (Widfield/Epifluorescence)
• Lumencor Celeste Light engine (Crest spinning disk confocal)
• Nikon 4-line LU-N4 (C2 Confocal)

Excitation Lasers (Crest Spinning Disk Confocal):

• 405 nm
• 446 nm
• 477 nm
• 520 nm
• 546 nm
• 638 nm
• 749 nm

Excitation Laser (C2 Confocal)

• 405nm
• 488nm
• 561nm
• 640nm