This spinning disk confocal system is ideal for high-speed, high-resolution live-cell imaging and is also well suited for fixed samples. It supports super-resolution imaging without requiring specialized fluorophores or extensive sample preparation. A digital micromirror device (DMD) enables precise photoconversion and photoactivation (e.g for optogenetics)

• High-speed spinning disk confocal imaging
• Live-cell and fixed-sample imaging
• Super-Resolution via Optical Re-assignment
• DMD-based targeted illumination
• Environmental control for live imaging (temperature and 5% CO₂)

Links

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Microscope Stand:

• Leica DMi8 Inverted Microscope with Adaptive Focus Control (AFC)
• ASI motorized stage with Piezo top plate (500 um travel)
• OkoLab enviromemental control incubator

Modules:

• Yokogawa CSU-W1 SoRa Confocal Scanner Unit (50um pinhole, SoRa disk, Mag changer (1, 2.8 and 4X) with Uniformizer
• Mightex Spatial Illuminator (combined with an LDI laser combiner)

Camera:

• Photometrics Kinetix Highspeed sCMOS camera

Light sources

• Vortran Laser combiner (4 lines for imaging)
  • 405, 488, 561, 639nm
• 89North LDI laser combiner (6 lines for photoactivation)
  • 405, 445, 470, 520, 528, 555, 640nm
• LED3 broadband epifluorescence light source
• White LED for transmitted light

Objectives:

• HC PL APO10x/0.45
• HC PL APO 20x/0.80
• HC PL APO 40x/0.95 CORR
• HCX PL APO 40x/1.10 W CORR
• HC PL APO 63x/1.40-0.60 OIL
• HC PL APO 100x/1.47 OIL CORR TIRF

Available upon request

• HC FLUOTAR L 25x/0.95 W VISIR
• HC PL FL10x/0.3 (11mm WD)Equipment Details:

Software

• MicroManager 2.0 Device control
• Microvolution live deconvolution software

Emission Filters:

• Chroma ET 460/50
• Chroma ET 605/70
• Chroma ET 700/75
• Chroma ET 525/50
• Chroma ZET405/488/561/635