CSU-W1 SoRa Spinning Disk Confocal Microscope

Ideal for confocal live cell imaging (great to image fixed samples as well) and super resolution without specific fluorophors or lengthy sample preparations. It also has a digital micromirror device (DMD) coupled with a 6 line laser combiner for simultaneous Photoconversion/Activation (optogenetics). It is equipped with environmental control (temperature and 5% Co2) for live imaging.

Links

iLab Calendar

Add FPbase link

Core WEbPage

 

Capabilities

  • High speed imaging (confocal and widefield)
  • Highspeed super resolution
  • Photoconversion/Activation with DMD
  • Live imaging

 

 

 

Objectives:

  • HC PL FL 10x/0.30
  • HC PL APO 20x/0.80
  • HC PL APO 40x/0.95 CORR
  • HCX PL APO 40x/1.10 W CORR
  • HC PL APO 100x/1.47 OIL CORR TIRF

Available upon request

  • HC FLUOTAR L 25x/0.95 W VISIR

 

Equipment Details:

  • Leica DMi8 Inverted Microscope with Adaptive Focus Control (AFC)
  • Yokogawa CSU-W1 SoRa Confocal Scanner Unit (50um pinhole, SoRa disk, Mag changer (1, 2.8 and 4X) with Uniformizer
  • Mightex Spatial Illuminator combined with an LDI laser combiner 
  • Photometrics Kinetix Highspeed sCMOS camera
  • ASI motorized stage with Piezo top plate (500 um travel)
  • OkoLab enviromemental control incubator
  • MicroManager Control and Microvolution live deconvolution software

Light Source:

  • Lumencore Spectra X for widefield

Excitation Filters/Wavelengths (lamp vs lasers)

  • Vortran Laser combiner (4 lines for imaging) 
    • 405, 488, 561, 639nm 
  • 89North LDI laser combiner (6 lines for photoactivation)
    • 405, 445, 470, 520, 528, 555, 640nm

Emission Filters:

For spinning disk confocal: 

  • Chroma ET 460/50
  • Chroma ET 605/70
  • Chroma ET 700/75
  • Chroma ET 525/50
  • Chroma ZET405/488/561/635 m

For Widefield

  • DAPI filter cube
  • FITC filter cube
  • Rhod filter cube
  • 405nm LP Dichroic for Mightex

 

Location: 
Weill Institute for Neurosciences Building (MB)
Room: 
451J
Core: 
Innovation Core (Weill)
Technique: 
Brightfield, DIC, phase
Confocal
FRAP/Photoactivation
HCA on Multi-well plates
High-speed imaging (>100 fps)
Inverted microscope
Live Cell
Super-resolution
Widefield epifluorescence
Staff contact: 
[email protected]
Special Access requirements: 
Laser Safety
Training by Core Staff