The Leica SP8 is an inverted laser scanning confocal system. The SP8 comes equipped with an AOBS tunable detection pathway (3 PMTs and 2 HyD), a white light laser (470nm-670nm). The HyD detectors offer a "true black" background and are able to gate on fluorescence lifetime (allowing for the in-line reduction of autofluorescence). The system also supports live imaging with a temperature-controlled environmental chamber.
The system also contains spectral scanning, three-dimensional (z) acquisition and time-lapse capabilities. A programmable stage allows automated return-to-site and multiple-field stitching. Software includes the Navigator module.
Capabilities
- Live Imaging
Objectives:
- HC PL APO CS 10x / 0.40, 2.20mm WD
- HC PL APO CS2 20x / 0.75, 0.66mm WD
- Fluotar VISIR 25x / 0.95, 2.10mm WD
- HC PL APO CS2 40x / 1.30, 0.24mm WD
- HC PL APO CS2 63x / 1.40, 0.14mm WD
- HC PL APO 100x / 1.44, 0.10mm WD
Equipment Details:
- Inverted configuration
- Environmental chamber (temp and CO2 control)
- White Light Laser
- 405nm Laser
- Tunable detectors (3 PMT, 2 HyD)
Light Source:
- Tunable white light laser (470nm-670nm)
Location:
Institute Regeneration Medicine (PH)
Room:
Pod B
Core:
BIDC
Type:
Light
Technique:
Confocal
Inverted microscope
Live Cell
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff