Keyence BZ-9000 ("Coyote 3")

The Coyote 3 is a Keyence BZ-9000 that is set up for automated widefield epifluorescence. The microscope can accommodate slides or 35mm dishes and can be used for imaging fixed samples, live samples (at room temperature), and tiling. large sample areas. Please note this microscope is not suited for long term imaging, thick samples (>50um), or plastic culture dishes.

 

Capabilities

  • Brightfield imaging
  • 4-Color Epifluorescence imaging
  • Tiling multiple images for large sample areas

 

 


 

Objectives:

  • Plan Apo 2x/0.1
  • Plan Apo 10x/0.45
  • Plan Apo 20x/0.75
  • S Plan Apo ELWD 20X/0.45
  • Plan Apo 40x/.95
  • Plan Apo 100/1.15

Equipment Details:

  • Keyence BZ-9000 microscope (Inverted)
  • 12-bit mono camera
  • 12-bit RGB camera
  • Metal halide illumination
  • BZ-X II software
  • Motorized stage

Light Source:

  • Metal halide illumination

Excitation Filters:

  • DAPI
  • GFP
  • TRITC
  • Cy5

Emission Filters:

  • DAPI
  • GFP
  • TRITC
  • Cy5

 

Location: 
Gladstone Institutes
Room: 
Gladstone- Bench 3M-1
Core: 
GHLMC
Type: 
Light
Technique: 
Brightfield, DIC, phase
Inverted microscope
Widefield epifluorescence
Special Access requirements: 
Laser Safety
Training by Core Staff