The Coyote 1 is a Keyence BZ-X700 that is set up for automated widefield epifluorescence with a high sensitivity RGB and monochrome camera. The microscope can accommodate slides, 35mm dishes, tissue culture plate and petri dishes. The machine can be used for imaging fixed samples, live samples (at room temperature), automated multi position and tiling, Structured illumination for increased resolution (pseudo confocal), phase contrast and oblique illumination. Please note this microscope is not suited for long term imaging or thick samples (>50um).
Capabilities
- Multipositional acquisition and tiling
- Structured illumination for increased resolution (pseudo-confocal)
- Phase contrast and oblique illumination for high contrast brightfield imaging
- 3D Imaging
- 4-color fluorescence
- Motorized stage
- Accommodates well-plates
Objectives:
- Plan Apo 2x/0.1
- Plan Fluor 4x/0.13
- Plan Apo 10x/0.45
- S Plan Fluor 20x/0.45
- S Plan Fluor 40x/.60
Equipment Details:
- Keyence BZ-X700 Microscope (Inverted)
- 14-bit/8-bit mono camera
- 8-bit RGB camera
- Metal halide illumination
- BZ-X Viewer Software
Light Source:
- Metal halide illumination
Excitation Filters/Wavelengths (lamp vs lasers)
- DAPI
- GFP
- TRITC
- Cy5
Emission Filters:
- DAPI
- GFP
- TRITC
- Cy5
Location:
Gladstone Institutes
Room:
Gladstone Rm. 452
Core:
GHLMC
Type:
Light
Technique:
Brightfield, DIC, phase
Inverted microscope
Widefield epifluorescence
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff