This system is a fully motorized stereomicroscope for both reflected and transmitted light. A high speed motorized stage enables large image acquisition and/or multi-position high throughput imaging of plates.
The system is equipped with a high speed (up to 30fps full frame) high sentivity camera combined with an LED for fluorescence illumination as well as both transmitted and reflected light for brightfield/oblique contrast imaging.
The system is ran by LASX software with a "Thunder" compuational clearing module.
It can also be ran in Micromanager/Pycromanager for custom scripts.
Equipment Details:
- Leica M205FCA stereo with motirized Z and filter turret
- Leica LMT260 XY-Scanningstage
- Hamamatsu Flash 4.0 v3
- LASX Software Package with Thunder computational clearing module
Objectives:
Plan Apo 1X objective
Plan Apo 2X objective with Corr Collar
Light Source:
- Leica LED 3 (390-680nm)
- White LEDs for transmitted and reflected light
Filter
- ET BFP - M205FA/M165FC filter cube
- ET GFP - M205FA/M1A/M165FC65FC
- ET mCherry - M205FA/M165FC filter cube
- ET CY5 - M205FA/M165FC filter cube
Location
Weill Institute for Neurosciences Building (MB)
Room
451J
Core
Innovation Core (Weill)
Type
Light
Technique
Brightfield, DIC, phase
Upright microscope
Widefield epifluorescence
Staff contact
Special Access requirements
Training by Core Staff