Summary:
This fully motorized stereomicroscope is designed for high-speed, large-area and multi-position imaging using fluorescence, transmitted, and reflected light. It combines a fast motorized stage and high-sensitivity camera with THUNDER computational clearing and support for custom acquisition workflows.
Capabilities
- High-speed stereomicroscopy
- Fluorescence, transmitted-light, and reflected-light imaging
- Large-area image acquisition and stitching
- Automated multi-position imaging
- High-sensitivity camera (up to 30 fps at full frame)
- THUNDER computational clearing
- Capacity for custom scripting for automation
Objectives:
- Plan Apo 1X objective
- Plan Apo 2X objective with Corr Collar
- Option for a Plan Apo 1.6X objective
Camera
- Hamamatsu Flash 4.0 v3 sCMOS camera
Software control
- Leica LASX for standard acquisition and computational clearing
- Micromanager/Pycomanager control to support for custom scripting and automation
Microscope Stand
- Leica M205FCA stereo with motorized Z and filter turret
- Leica LMT260 XY-Scanningstage
Light Source:
- Leica LED 3 (390-680nm)
- White LEDs for transmitted and reflected light
Filters
- ET BFP - M205FA/M165FC filter cube
- ET GFP - M205FA/M1A/M165FC65FC
- ET mCherry - M205FA/M165FC filter cube
- ET CY5 - M205FA/M165FC filter cube
Location
Weill Institute for Neurosciences Building (MB)
Room
451J
Core
Innovation Core (Weill)
Type
Light
Technique
Brightfield, DIC, phase
Upright microscope
Widefield epifluorescence
Staff contact
Special Access requirements
Training by Core Staff