The Leica SP8 is an upright laser scanning confocal system configured for large volume (cleared tissue) multiplexed imaging. The SP8 comes equipped with an AOBS tunable detection pathway (3 PMTs and 2 HyD), a white light laser (470nm-670nm), and the Lighting deconvolution module. The HyD detectors offer a "true black" background and are able to gate on fluorescence lifetime (allowing for the in-line reduction of autofluorescence).
The system also contains spectral scanning, three-dimensional (z) acquisition and time-lapse capabilities. A programmable stage allows automated return-to-site and multiple-field stitching. Software includes the Navigator module.
Capabilities
- Large volume, cleared tissue imaging
Objectives:
- Fluotar (BABB) 16x
- Fluotar IMM MotCorr 16x
- IMM HC PL APO CS2 16x
- Apo IMM (H2O Dipping) 20x
- Fluotar IMM MotCorr 25x
- HCX Plan Apochromat H20
Equipment Details:
- Upright configuration
- White Light Laser
- 405nm Laser
- Tunable detectors (3 PMT, 2 HyD)
Light Source:
- Tunable white light laser (470nm-670nm)
Location:
Health Sciences West (PH)
Core:
BIDC
Type:
Light
Technique:
Confocal
Upright microscope
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff