Laser-scanning confocal with a pulsed White-Light Laser (440-790nm), 5 photon counting high sensitivity detectors and 3 STED depletion lasers. On this system you can do standard laser-scanning confocal microscopy, multichannel super resolution by stimulated emission depletion (STED and Tau-STED), Fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime imaging (FLIM, FCS, FLIM-FRET...). It is equipped with environmental control (temperature and 5% Co2) for live imaging.
Capabilities
- Laser scanning Confocal
- STED (stimulated emision depletion) based super resolution
- FLIM (Fluorescence LIfetime Imaging)
Objectives:
- HC PL APO 10x/0.4 NA CS2
- HC PL APO 20x/0.75 NA CS2
- HC PL APO 40x/1.30 Oil CS2
- HC PL APO 86x/1.20 W motCORR STED W
- PHC PL APO 100x/1.40 OIL STED W
Available upon request - HC FLUOTAR L 25x/0.95 W VISIR
- HC PL APO 93x/1.30 GLYC motC STED W
- HC PL APO 40x/0.95 CORR
Light Source:
- Leica LED 3 (390-680nm) with 3mm LLG for widefield
Equipment Details:
- Leica DMi8 Inverted Microscope with Adaptive Focus Control (AFC)
- Scanning motorized stage and Z-Piezo stage with 500um travel (WieneckeSinske WSBPiezo)
- 8kHz Tandem Scanner STELLARIS 8 (galvo and resonant)
- HyD detectors
- HyD S (pos 1 and pos 3)
- HyD X (pos 2 and pos 4)
- HyD R (pos 5)
- Lasers:
- 405nm CW
- White Light Laser (pulsed, 440 to 790 nm)
- 592 nm STED depletion laser
- 660 nm STED depletion laser
- 775 nm STED depletion laser
Location:
Weill Institute for Neurosciences Building (MB)
Room:
451J
Core:
Innovation Core (Weill)
Technique:
Brightfield, DIC, phase
Confocal
FLIM
FRAP/Photoactivation
Inverted microscope
Live Cell
Spectral imaging
Super-resolution
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff