This laser-scanning confocal microscope combines a pulsed white-light laser (440–790 nm), five high-sensitivity photon-counting detectors, and three STED depletion lasers for advanced confocal, super-resolution, and fluorescence lifetime imaging. The system supports both fixed-sample and live-cell applications, with environmental control for temperature and 5% CO₂.

• Laser-scanning confocal microscopy
• Multichannel STED and Tau-STED super-resolution imaging
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence lifetime imaging microscopy (FLIM)
• Fluorescence correlation spectroscopy (FCS)
• FLIM-FRET
• Lamda scanning (Ex and Em) and Spectral unmixing
• Live-cell imaging with temperature and 5% CO₂ control

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Microscope Stand

• Leica DMi8 Inverted Microscope with Adaptive Focus Control (AFC)
• Scanning motorized stage and Z-Piezo stage with 500um travel (WieneckeSinske WSBPiezo)

Objectives:

• HC PL APO 10x/0.4 NA CS2
• HC PL APO 20x/0.75 NA CS2
• HC PL APO 40x/1.30 Oil CS2
• HC PL APO 86x/1.20 W motCORR STED W
• PHC PL APO 100x/1.40 OIL STED W
  
  Available upon request
• HC FLUOTAR L 25x/0.95 W VISIR
• HC PL APO 93x/1.30 GLYC motC STED W
• HC PL APO 40x/0.95 CORR

Light Sources:

• LED 3 broadband (390-680nm) with 3mm LLG for widefield
• White LED for transmitted light/DIC

Confocal module

• 8kHz Tandem Scanner STELLARIS 8 (galvo and resonant)
• HyD detectors:
  • HyD S (pos 1 and pos 3)
  • HyD X (pos 2 and pos 4)
  • HyD R (pos 5)

Lasers

• 405nm CW laser
• 440-790nm White Light Laser (pulsed 10 to 80Hz)
• 592 nm STED depletion laser
• 660 nm STED depletion laser
• 775 nm STED depletion laser