This system is a fully motorized high speed widefield imaging microscope for both reflected and transmitted light (fluorescence, brightfield and polarized light). A high speed motorized stage enables large image acquisition and multi-position high throughput imaging of plates.
The system is equipped with a high speed (up to 100fps full frame) high sentivity camera combined with an 8 channel LED with sequencer for high speed imaging of both fixed and live samples. It is also equipped with a color camera for imaging of both colorimetrics dyes (H&E etc...) as well as polarized light tissue stains.
The system is ran by LASX software with a "Thunder" compuational clearing module.
Finally, It is equipped with environmental control (temperature and 5% Co2) for live imaging.
Equipment Details:
- Leica DMi8 Inverted Microscope with Adaptive Focus Control (AFC)
- Quantum HS linear motorized stage (PECON)
- Hamamatsu Flash 4.0 v3 with CAM-Link (100fps full frame)
- Leica DFCcolor camera
- OkoLab Cage Incubator
- LASX Software Package with Thunder computational clearing module
Objectives:
- N PLAN 5x/0.12 PH0
- HC PL APO 10x/0.45
- HC PL APO 20x/0.80
- HC PL APO 40x/0.95 CORR
- HC PL APO 63x/1.40-0.60 OIL
Light Source:
- Leica LED 8 with sequencer (395, 438, 475, 511, 555, 575, 635, 730 nm)
- White LED for transmitted light
Filter cubes
"DAPI"
- EX: 375-435
- DC: 455
- EM: 450-490
"GFP"
- EX: 450-490
- DC: 495
- EM: 500-550
Quad for CFP-YFP-RFP and Cy7 (CYR71010)
- EX: 422-450; 495-517; 566-590; 710-750
- DC: 459; 523; 598; 763
- EM: 462-484; 527-551; 602-680; 770-850
Quad for DAPI-FITC-TRITC and Cy5 (DFT51010)
- EX: 375-407; 462-496;542-566; 622-654
- DC: 415; 500; 572; 660
- EM: 420-450; 506-532; 578-610; 666-724
Analyser for polarized light.
Emission Filter wheel:
- 460/80
- 535/70
- 590/50
- 642/80
- 100%