This fully motorized inverted microscope is designed for fast, versatile imaging. It features a high-speed stage, two cameras, and a triggerable multichannel LED illumination system.

• High-speed widefield imaging
• Automated tiled imaging and image stitching for large-area acquisition.
• Epifluorescence, transmitted-light, and polarized-light imaging
• Monochrome and color cameras
• Computational clearing for “pseudo-confocal” imaging (Leica THUNDER)
• Live-cell imaging with temperature and gas control

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Microscope:

• Leica DMi8 Inverted Microscope with Adaptive Focus Control (AFC)
• Quantum High speed  linear motorized stage (PECON)
• OkoLab Cage Incubator

Objectives:

• N PLAN 5x/0.12 PH0
• HC PL APO 10x/0.45
• HC PL APO 20x/0.80
• HC PL APO 40x/0.95 CORR
• HC PL APO 63x/1.40-0.60 OIL

Cameras:

• Hamamatsu Flash 4.0 v3 with CAM-Link (100fps full frame)
• Leica DMC4500 digital color camera 

Light Source:

• Leica LED 8 with sequencer (395, 438, 475, 511, 555, 575, 635, 730 nm)
• White LED for transmitted light 

Software:

• LASX Software Package
• Thunder computational clearing module

Filter cubes

• Quad for CFP-YFP-RFP and Cy7 (CYR71010)
  • EX: 422-450; 495-517; 566-590; 710-750
  • DC: 459; 523; 598; 763
  • EM: 462-484; 527-551; 602-680; 770-850
• Quad for DAPI-FITC-TRITC and Cy5 (DFT51010)
  • EX: 375-407; 462-496;542-566; 622-654
  • DC: 415; 500; 572; 660
  • EM: 420-450; 506-532; 578-610; 666-724
• Analyser for polarized light.

Emission Filter wheel:

• 460/80
• 535/70
• 590/50
• 642/80
• 100%