
The SmartSPIM is a lightsheet microscope offering uniform axial resolution across the whole sample and high speed volumetric imaging.
It can handle fixed samples ranging in size from a couple milimeters up to 40x75mm.
It is compatible with multiple imaging solutions to adapt to many clearing methods with a very simple and rapid alignment procedure. Three imaging modes: high speed (30fps), regular (20fps) and high sensitivity (5fps and 5x exposure).
Imaging uses axially swept lightsheet for Uniform Axial resolution as well as focus compensation for sample refractive-index mismatching and chromatic focal shift.
Dual computer set up for simulatenous acquisition and processing, very intuitive and simple imaging software
Capabilities
- High-speed fixed sample imaging with a high sensititvity camera
- Ability to image large, thick samples at high resolution with low photobleaching
- Tissue Clearing Imaging (acquous and organic solvants)
- Whole tissue, organoid, organism and small organism imaging
Objectives: MultiImmersion Dipping Objectives
- 1.63X
- 3.6X
- 9X
Equipment Details:
- LifeCanvas SmartSPIM Lightsheet Micorscope
- Hamamatsu ORCA Fusion camera
- Vortran Stradus Versalase Laser combiner
Laser Lines:
- 488nm
- 561nm
- 639nm
- 785nm
Emission Filters
- 525/20nm
- 600/50nm
- 690/50nm
- 792nm LP