The SmartSPIM is a lightsheet microscope offering uniform axial resolution across the whole sample and high speed volumetric imaging.
It can handle fixed samples ranging in size from a couple milimeters up to 40x75mm.
It is compatible with multiple imaging solutions to adapt to many clearing methods with a very simple and rapid alignment procedure. At this point we offer Easy Index Imaging Oil (1.52 for SHIELD and CUBIC) and EthylCinamate (1.56 for iDisco..)
Imaging uses axially swept lightsheet for Uniform Axial resolution as well as focus compensation for sample refractive-index mismatching and chromatic focal shift.
Dual computer set up for simulatenous acquisition and processing, very intuitive and simple imaging software.
Capabilities
- High-speed fixed sample imaging with a high sensititvity camera
- Ability to image large, thick samples at high resolution with low photobleaching
- Compatible with various tissue clearing protocols (acquous and organic solvants)
- Whole tissue, organoid, organism and small organism imaging
Objectives: MultiImmersion Dipping Objectives
- 1.63X (resulting 4um voxel)
- 3.6X (resulting 1.8um voxel)
- 9X (resulting 0.7um voxel)
Equipment Details:
- LifeCanvas SmartSPIM Lightsheet Micorscope
- Hamamatsu ORCA Fusion camera
- Vortran Stradus Versalase Laser combiner
Laser Lines:
- 488nm
- 561nm
- 639nm
- 785nm
Emission Filters
- 525/20nm
- 600/50nm
- 690/50nm
- 792nm LP