The SmartSPIM is a light-sheet microscope designed for high-speed volumetric imaging of large, optically cleared samples. It uses an axially swept light sheet to provide uniform axial resolution across the field of view, with focus compensation for refractive-index mismatch and chromatic focal shifts.
The system accommodates fixed samples ranging from a few millimeters up to 40 × 75 mm and is compatible with multiple tissue-clearing methods. Available imaging media include EasyIndex Imaging Oil (RI 1.52) for protocols such as SHIELD and CUBIC, and ethyl cinnamate (RI 1.56) for iDISCO and related methods.
A dual-computer setup enables simultaneous image acquisition and processing for efficient high-volume imaging workflows.
- High-speed volumetric light-sheet imaging
- High-sensitivity camera with low photobleaching
- Axially swept light sheet for uniform axial resolution
- Refractive-index mismatch and chromatic focal-shift compensation
- Simultaneous image acquisition and processing
Objectives (Multi-immersion)
- 1.63X (resulting 4um voxel)
- 3.6X (resulting 1.8um voxel)
- 9X (resulting 0.7um voxel)
Microscope
- LifeCanvas SmartSPIM Lightsheet Micorscope
- Hamamatsu ORCA Fusion camera
- Vortran Stradus Versalase Laser combiner
Laser Lines:
- 488nm
- 561nm
- 639nm
- 785nm
Emission Filters
- 525/20nm
- 600/50nm
- 690/50nm
- 792nm LP
Location
Weill Institute for Neurosciences Building (MB)
Room
Room 472A
Core
Innovation Core (Weill)
Type
Light
Technique
High-speed imaging (>100 fps)
Lightsheet
Staff contact
Special Access requirements
Laser Safety
Training by Core Staff
Grants/Funding to be acknowledged
Sandler Program for Breakthrough Biomedical Research grant, which is partially funded by the Sandler Foundation.