LifeCanvas SmartSPIM Highspeed Lightsheet Microscope

The SmartSPIM is a lightsheet microscope offering uniform axial resolution across the whole sample and high speed volumetric imaging.

It can handle fixed samples ranging in size from a couple milimeters up to 40x75mm.

It is compatible with multiple imaging solutions to adapt to many clearing methods with a very simple and rapid alignment procedure. Three imaging modes: high speed (30fps), regular (20fps) and high sensitivity (5fps and 5x exposure).

Imaging uses axially swept lightsheet for Uniform Axial resolution as well as focus compensation for sample refractive-index mismatching and chromatic focal shift.

Dual computer set up for simulatenous acquisition and processing, very intuitive and simple imaging software
 

 

Capabilities

  • High-speed fixed sample imaging with a high sensititvity camera
  • Ability to image large, thick samples at high resolution with low photobleaching
  • Tissue Clearing Imaging (acquous and organic solvants)
  • Whole tissue, organoid, organism and small organism imaging

 

 


 

Objectives: MultiImmersion Dipping Objectives

  • 1.63X
  • 3.6X
  • 9X

Equipment Details:

  • LifeCanvas SmartSPIM Lightsheet Micorscope
  • Hamamatsu ORCA Fusion camera
  • Vortran Stradus Versalase Laser combiner

Laser Lines:

  • 488nm
  • 561nm
  • 639nm
  • 785nm

Emission Filters

  • 525/20nm
  • 600/50nm
  • 690/50nm
  • 792nm LP 

 

Location: 
Weill Institute for Neurosciences Building (MB)
Room: 
Room 472A
Core: 
Innovation Core (Weill)
Type: 
Light
Technique: 
High-speed imaging (>100 fps)
Lightsheet
Staff contact: 
Special Access requirements: 
Laser Safety
Training by Core Staff
Grants/Funding to be acknowledged: 
Sandler Program for Breakthrough Biomedical Research grant, which is partially funded by the Sandler Foundation.