The ImageXpress HT.ai is a high-content imaging system designed for automated, high-throughput imaging of multi-well plates. It provides both spinning-disk confocal and widefield imaging and is equipped with air and water-immersion objectives. Environmental control of temperature and CO₂ enables extended live-cell imaging experiments.

• High-content, high-throughput imaging
• Spinning-disk confocal (pinhole and slit) and widefield imaging
• Automated multi-well plate imaging
• Air and water-immersion objectives
• Live-cell imaging with temperature and CO₂ control

Links

request training

Add FPbase link

Core WEbPage

Objectives:

• Plan Apo Lambda 2x/0.1NA (default overview objective)
• Plan Apo 10x/0.45NA
• Apo LWD 20x/0.95NA WI
• Apo LWD 40x/1.15NA WI

Option: Plan Apo Lambda 4x/0.2NA 

Illumination

• White LED for transmitted light 
• Two 89 North LDI laser combiners for a total of 7 laser lines (405, 445, 475, 520, 555, 640 and 730nm) 

Camera

Andor Zyla 4.2 sCMOS camera

Spinning disk unit

• Dual disk unit allowing selection between 3 modes
  1. 60 micron pinhole disk for your basic confocal requirements
  2. 50 micron slit for high throughput confocal requirements
  3. widefield imaging mode (bypass)

Software

Metaxpress acquisition and analysis

Filters:

Dichroics:

1. FF409-Di03 (409 nm LP)
2. FF506-Di03 (506nm LP)
3. FF593-Di03-25 (593nm LP)
4. ZT 445/520 (dual BP)
5. ZT 405/470/555/640/730 (penta)

Excitation filters

• 445/20 nm
• 467.5/21 nm
• 520/10 nm
• 555/X nm
• 638/17 nm
• 725/30 nm

Emission filters

• 483/32 nm
• 520/28 nm
• 562/40 nm
• 598/25 nm
• 598/25 nm
• 692/40 nm