Nikon A1R

The Nikon A1R is an upright laser scanning confocal microscope capable of reaching video rates. The microscope consists of two illumination options; four confocal laser lines (408 nm, 488 nm, 561 nm, 647 nm) and a Mai Tai DeepSee IR laser (740 - 1000 nm). For the confocal laser lines there are 4 PMT detectors (blue, green, red, far red), and for the two-photon imaging there is a high sensitivity non-descanned detector (NDD) for deep tissue imaging.

The system utilizes a Nikon base in the upright geometry, an automated Prior XY stage, and a PIFOC objective piezo for scanning. Additionally, there is a perfusion system with an in-line stage heater and an anesthesia setup making the microscope suitable for slide, slice, explant, and intravital imaging.

 

Capabilities

  • Microscope capabilities

 

 


 

Objectives:

  • Plan Apochromat LWD H20 16x
  • XLUM PlanFl LWD H20 20x
  • Apo LWD H20 25x
  • Plan Apochromat VC H20 60x
  • Plan Apochromat VC Oil 60x

 

 


Light Source:

  • OBIS 405nm laser
  • OBIS 488nm laser
  • OBIS 561nm laser
  • OBIS 647nm laser
  • Tunable SpectraPhysics Infrared Laser (740-1000nm)

 

 

 

Location: 
Medical Sciences Building (PH)
Room: 
S1121
Core: 
BIDC
Type: 
Light
Technique: 
Confocal
Live Cell
Multi-photon
Upright microscope
Staff contact: 
Special Access requirements: 
Laser Safety
Training by Core Staff