Nikon CSU-X1 Spinning Disk

Nikon CSU-X1 Spinning Disk

This is an inverted Nikon Ti microscope equipped with a CSU-X1 spinning disk confocal, Photometrics Prime 95B sCMOS camera, and an OkoLab cage incubator with CO2 and humidity control. The system is optimized for imaging live cells with high sensitivity and resolution. The Nikon Perfect Focus System can be used to keep live samples in focus over time and at multiple positions.

 

Capabilities

  • 3D confocal imaging
  • 4-color fluorescence imaging
  • Live cell time lapse imaging
  • Automated multi-position imaging
  • Stimulation with 440nm 630nm though brightfield light path

 

 


 

Objectives:

  • Plan Apo λ 10x / 0.45
  • Plan Apo VC 20x/0.75
  • Plan Apo λ 40x/0.95
  • Plan Apo λ 40x/1.3 oil
  • Plan Apo VC 100X/1.4
  • Apo TIRF 100x/1.49 Oil

 

Equipment Details:

  • Nikon Ti Microscope (Inverted)
  • Yokogawa CSU-X1 Spinning Disk Confocal
  • Vortran 4-line laser launch
  • Sutter Lambda XL
  • Photometrics Prime95B sCMOS camera
  • Andor DU897 EMCCD
  • Sutter Lambda 10-3 emission filter wheel
  • ASI motorized XY stage with Z piezo
  • OkoLab Cage Incubator
  • OkoLab CO2 Mixer

Light Source:

  • Vortran 4-line solid state laser launch

 

Excitation Wavelengths:

  • 405nm
  • 488nm
  • 561nm
  • 640nm

Emission Filters:

  • 460/50m
  • 525/50m
  • 630/75m
  • 690/50m

 

Location: 
Smith Cardiovascular Research Building
Room: 
CVRI 382B
Core: 
CALM (CVRI-MRC)
Type: 
Light
Technique: 
Brightfield, DIC, phase
Confocal
Inverted microscope
Live Cell
Staff contact: 
Kari Herrington, SoYeon Kim
Special Access requirements: 
Laser Safety
CVRI PIs only
Training by Core Staff
Grants/Funding to be acknowledged: 
UCSF Research Evaluation and Allocation Committee, the Gross Fund, and the Heart Anonymous Fund.