The Nikon Widefield Epifluorescence microscope is an inverted Nikon Ti microscope set up for 4-color fluorescence imaging for both fixed and live cells. The microscope is equipped with an Andor Clara interline CCD, an In Vivo Scientific cage incubator, and an Oko lab CO2 mixer.
Capabilities
- Bright field and DIC imaging
- 4-color fluorescence imaging
- Live cell imaging
- Automated imaging
Objectives:
- Plan Apo 4x/0.2
- Plan Fluor 10x/0.3
- Plan Apo VC 0.75NA 20x
- Plan Apo 20X ELWD
Equipment Details:
- Nikon Ti Inverted Microscope
- Andor Clara Interline CCD
- In Vivo Scientific Cage incubator
- Oko CO2 Mixer
- Sutter Lambda XL lamp
Light Source:
- Sutter Lambda XL lamp
Excitation Filters
- DAPI (340-380nm)
- FITC (465-495nm)
- TRITC (528-553nm)
- Cy5 (625-650nm)
- mCherry LP (542-582nm)
Emission Filters:
- DAPI (435-485nm)
- FITC (515-555nm)
- TRITC (590-650nm)
- Cy5 (peak 670)
- mCherry-LP (604-1100nm)
Location
Smith Cardiovascular Research Building (MB)
Room
CVRI 382B
Core
CALM (CVRI-MRC)
Type
Light
Technique
Brightfield, DIC, phase
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact
Special Access requirements
Laser Safety
Training by Core Staff