Nikon Widefield Epifluorescence

The Nikon Widefield Epifluorescence microscope is an inverted Nikon Ti microscope set up for 4-color fluorescence imaging for both fixed and live cells. The microscope is equipped with an Andor Clara interline CCD, an In Vivo Scientific cage incubator, and an Oko lab CO2 mixer.

 

Capabilities

  • Bright field and DIC imaging
  • 4-color fluorescence imaging
  • Live cell imaging
  • Automated imaging

 

 


 

Objectives:

  • Plan Apo 4x/0.2
  • Plan Fluor 10x/0.3
  • Plan Apo VC 0.75NA 20x
  • Plan Apo 20X ELWD

 

Equipment Details:

  • Nikon Ti  Inverted Microscope
  • Andor Clara Interline CCD
  • In Vivo Scientific Cage incubator
  • Oko CO2 Mixer
  • Sutter Lambda XL lamp

Light Source:

  • Sutter Lambda XL lamp

Excitation Filters

  • DAPI (340-380nm)
  • FITC (465-495nm) 
  • TRITC (528-553nm)
  • Cy5 (625-650nm)
  • mCherry LP (542-582nm)

Emission Filters:

  • DAPI (435-485nm)
  • FITC (515-555nm)
  • TRITC (590-650nm)
  • Cy5 (peak 670)
  • mCherry-LP (604-1100nm)

 

Location: 
Smith Cardiovascular Research Building
Room: 
CVRI 382B
Core: 
CALM (CVRI-MRC)
Type: 
Light
Technique: 
Brightfield, DIC, phase
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact: 
Kari Herrington, SoYeon Kim
Special Access requirements: 
Laser Safety
CVRI PIs only
Training by Core Staff