The Snow Leopard is an inverted Olympus FV3000RS point scanning confocal. The microscope is equipped with a gas chamber with perfusion capabilities and an incubation chamber with temperature, CO2 control and humidity control. The microscopes utilize a TruFocus system with Z drift correction for imaging over time and has a variety of slide holders ranging from chamber slides, to 35mm dishes, and a multiwell plate holder.
Capabilities
- Scanning confocal imaging for live and fixed -cell, organoid and tissue imaging
- Live-cell fluorescence dynamics (i.e. FRAP, FRET, GCaMP)
- Imaging individual cells at high resolution, any magnification
- Multipositional acquisition and tiling multiple images for large sample areas
Objectives:
- 4X UPLXAPO (NA= .16)
- 10X UPLSAPO (NA= .4)
- 20X UPLSAPO (NA = .75)
- 40X UPLXAPO (NA = .95)
- 60X oil UPLSAPO (NA = 1.35)
Equipment Details:
- Olympus FV3000 (inverted)
- Both Galvo & Resonance scanning
- Cooled GaAsP photomultiplier, 2 channels
- TruFocus with Z drift correction function
- Motorized (X,Y,Z) stage
- Incubation chamber (CO2, Temperature & Humidity)
Light Source:
- Olympus Laser Launch
Excitation Wavelengths (lamp vs lasers)
- 405nm
- 445nm
- 488nm
- 514nm
- 594nm
- 561nm
- 638nm
Emission Filters:
- Spectral Detector
Location:
Gladstone Institutes
Room:
Gladstone
Core:
GHLMC
Type:
Light
Technique:
Confocal
FRAP/Photoactivation
High-speed imaging (>100 fps)
Inverted microscope
Live Cell
Spectral imaging
Super-resolution
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff