Operetta High Throughput Imager

Perkin Elmer Operetta High Throughput High Content Imager

The PerkinElmer Operetta is a high-content fluorescent microscope optimized for quickly imaging multiwall plates (eg. 96, 384, etc).  It is great for experiments in which you want to analyze hundreds or thousands of different conditions or to look at a few live or fixed samples in great detail.  Outputs can be as simple as cell number, or can include complex morphologic phenotypes or any fluorescent reporter.

 

Capabilities

  • Multichannel fluorescence 
  • Digital phase contrast bright field imaging
  • Multiposition acquisition 
  • High Troughput 
  • High Content

 

 


 

Objectives:

  • 10x NA 0.4 WD 3.21mm 
  • 20x NA 0.45 WD 7.80mm
  • 40x NA 0.95 WD 0.29mm

 

Equipment Details:

  • Perkin Elmer Operetta 
  • Excelitas Cemax Xenon
  • Operating and analysis software Harmony 3.4 

Light Source:

  • Excelitas Cemax Xenon

Excitation Filters:

  • 360-400nm
  • 410-430nm
  • 460-490nm
  • 490-510nm
  • 520-550nm
  • 560-580nm
  • 600-630nm
  • 620-640nm

Emission Filters:

  • 410-480nm
  • 500-550nm
  • 560-630nm
  • 650 LP

 

Location: 
Helen Diller
Room: 
HD 337
Core: 
LCA
Type: 
Light
Technique: 
Brightfield, DIC, phase
HCA on Multi-well plates
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact: 
Anna Celli, Ben Braun
Grants/Funding to be acknowledged: 
NIH P30CA082103