The Lightsheet Ultra Microscope Blaze from Miltenyi Biotec can be used with aqueous buffers and organic solvents and is equipped with a sCMOS camera. The system can be used for imaging large thick samples at high resolution with low photobleaching and phototoxicity. This machine includes lightspeed mode, allowing for faster imaging and a large imaging chamber for samples as small as organoids to as large as mice!
Capabilities
- Imaging (multiple) small to large samples in 3D with low photobleaching and high relative speed
- Pre-cleared sample (aqueous buffers and organic solvents with RI 1.33 – 1.57)
- Organoids to adult mouse
Objectives:
- 1.1X and 4X (0.6X – 10X) total range
Equipment Details:
- Camera: 4.2 Mpx sCMOS (2048 x 2048 pixels)
- Up to 6 light sheets (3 x 2 dual side) for optical sectioning
- Adjustable light sheet thickness: 4 – 24 μm (NA 0.0135 – 0.135)
- *Z-range constrained by objective working distance (≥16 mm for 1.1X, ≥15 mm for 4X)
Excitation Filters/Wavelengths (lamp vs lasers)
- 405nm
- 488nm
- 561nm
- 639nm
- 785nm
Location:
Gladstone Institutes
Room:
Gladstone Rm. 326
Core:
GHLMC
Type:
Light
Technique:
Lightsheet
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff