The Tortoise is a Zeiss AxioObserver A1 Inverted widefield fluorescence microscope capable of 4-Channel imaging. The microscope is wide-field epifluorescence system for fixed and live-cell imaging or imaging brightfield with a polarizing filter to capture birefringence. The microscope is equipped with a motorized stage, temperature and CO2 control, and Zeiss’s Definite Focus system for maintaining focus over time.
Capabilities
- Long-term live-cell imaging experiments
- Multipositional acquisition and tiling multiple images for large sample areas
- Polarizing filter for imaging birefringence
Objectives:
- 2.5X
- 5X
- 10X
- 20X
- 40X
- 63X oil
Equipment Details:
- Zeiss AxioObserver A1 (Inverted) Microscope
- Metal halide illumination
- Motorized stage,
- Incubation chamber and CO2 control
- Polarizing filter for imaging birefringence
- Axiocam MRm Camera
- Zen Blue Software
Light Source:
- Metal halide illumination
Excitation Filters/Wavelengths (lamp vs lasers)
- DAPI
- GFP
- RFP
- mCherry
- Cy5
Emission Filters:
- DAPI
- GFP
- RFP
- mCherry
- Cy5
Location:
Gladstone Institutes
Room:
Gladstone Rm. 432
Core:
GHLMC
Type:
Light
Technique:
Brightfield, DIC, phase
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff