The Cuttlefish is a Zeiss AxioObserver Z1 Inverted widefield fluorescence microscope capable of 4-Channel imaging. The microscope is wide-field epifluorescence system for fixed and live-cell imaging or imaging brightfield. The microscope is equipped with a motorized stage, temperature and CO2 control, and Hamamatsu Flash 4.0 sCMOS camera for high speed imaging.
Capabilities
- 4-Color Epifluorescence imaging
- Live chamber with temperature and CO2 control
- Time-lapse
- Multipositional acquisition and tiling multiple images for large sample areas
Objectives:
- 2.5X
- 5X
- 10X
- 20X
- 40X
- 63X (oil)
Equipment Details:
- Zeiss AxioObserver Z1 (inverted) Microscope
- Motorized stage
- Incubation Chamber (temperature and CO2 control)
- Hamamatsu Flash4.0 sCMOS camera
Light Source:
- Metal halide illumination
Excitation Wavelengths:
- DAPI
- GFP
- RFP
- Cy5
- mCherry
Emission Filters:
- DAPI
- GFP
- RFP
- Cy5
- mCherry
Location:
Gladstone Institutes
Room:
Gladstone Rm. 461
Core:
GHLMC
Type:
Light
Technique:
Brightfield, DIC, phase
High-speed imaging (>100 fps)
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff