This spinning disk confocal microscope features full environmental control and focus control for long time-lapse multichannel live cell and tissue imaging, software controlled motorized stage and objective turret for large area tiling and multi position/well acquisition and z-stacking.
Capabilities
- Time lapse imaging
- Full environmental control
- z-stack
- Multichannel acquisition
- Multiposition acquisition
- tiling
Objectives:
- Plan-Neofluar 2.5x
- APlan Apo 10x N.A. 0.45
- Plan Apo 20x N.A. 0.85
- Plan-Neo 40x N.A. 0.6
- Plan Apo 63X Oil N.A. 1.47
- Alfa Plan Apo 100X Oil N.A. 1.5
Equipment Details:
- Zeiss Axio imager
- Yokagawa CSU X1 spinning disk module
- Evolve EMCCD camera on the confocal arm
- AxioCam 506M on the epifluorescence arm
- Zeiss ZEN 2012 Acquisition software
Light Source:
- Excellitas X-Cite 120Q
- Zeiss Laser Launch
Excitation Wavelengths (Lasers):
- 405nm
- 488nm
- 561nm
Emission Filters:
- DAPI
- GFP
- mCherry
- Cy5
Location:
Helen Diller
Room:
HD337
Core:
LCA
Technique:
Brightfield, DIC, phase
Confocal
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact:
Anna Celli
Special Access requirements:
Training by Core Staff
Grants/Funding to be acknowledged:
NIH P30CA082103