The Tarsier is a Zeiss LSM 880 is a point-scanning confocal microscope equipped with a an airyscan for super resolution microscopy with resolutions up to 140nm in XY and 400nm in Z. The microscope is equipped with a live cage incubator, an objective inverter for using dipping lenses, and Zeiss’s definite focus system for keeping in focus over time. The microscope can be used for imaging speed up to 13fps to capture live cell dynamics (i.e. FRET, FRAP, and GCaMP), and multipositional acquisition or tiling.
Capabilities
- Scanning confocal imaging for mid-speed (13 fps) live-cell imaging
- Live-cell fluorescent dynamics (i.e. FRAP, FRET, GCaMP)
- Imaging individual cells at high resolution, any magnification
- GaSAP specrtal detection
- Multipositional aquisition and tiling multiple images for large sample area
Objectives:
- 5X
- 10X
- 20X
- 40XW
- 63XO
- 40X (air)
Equipment Details:
- Zeiss LSM 880 microscope (Inverted)
- Airyscan detector
- Zeiss Laser Launch
- GaASP detector
- Definite focus
- Objective inverter for using dipping lenses
- Cage incubator
Light Source:
- Zeiss Laser Launch
Excitation Wavelengths:
- 405nm
- 455nm
- 488nm
- 514nm
- 561nm
- 638nm
Emission:
- Spectral detection with variable beam splitter dichroics
Location:
Gladstone Institutes
Room:
Gladstone Rm. 361
Core:
GHLMC
Type:
Light
Technique:
Brightfield, DIC, phase
Confocal
FRAP/Photoactivation
Inverted microscope
Live Cell
Spectral imaging
Super-resolution
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff