The Zeiss PALM Laser Microdissection system offers contact free manipulation and microdissection of tissues and cell culture system. The separation/manipulation of the specimen occurs by means of a laser beam focused precisely and with high accuracy on a user selected region of interest. This is a tool for isolation of single cells or cell groups in preparation for PCA, RT-PCR, and proteomics.
Capabilities
- Microdisection and microcapture
- Touch Cap collection
- Fluorescence and brigthfield imaging
- Precise scratch assays
Objectives:
- 5x Fluar
- 20x LD Plan Neo
- 40x LD Plan Neo
- 63x Plan Neo
Equipment Details:
- Zeiss Axio Observer
- Zeiss PALM LMD dissection module
- Zeiss AxioCam
- Zeiss Palm Robot acquisition software
Light Source:
- Dissection laser: UV
- Imaging: Excellitas X-Cite 120Q
Filter cubes:
- DAPI
- GFP
- Rhodamine
- Cy5
Location:
Helen Diller
Room:
HD 355
Core:
LCA
Technique:
Brightfield, DIC, phase
Inverted microscope
Live Cell
Microdissection
Widefield epifluorescence
Staff contact:
Sarah Elmes
Special Access requirements:
Laser Safety
Training by Core Staff