The Zeiss PALM Laser Microdissection system offers contact free manipulation and microdissection of tissues and cell culture system. The separation/manipulation of the specimen occurs by means of a laser beam focused precisely and with high accuracy on a user selected region of interest. This is a tool for isolation of single cells or cell groups in preparation for PCA, RT-PCR, and proteomics.
Capabilities
- Microdisection and microcapture
- Touch Cap collection
- Fluorescence and brigthfield imaging
- Precise scratch assays
Objectives:
- 5x Fluar
- 20x LD Plan Neo
- 40x LD Plan Neo
- 63x Plan Neo
Equipment Details:
- Zeiss Axio Observer
- Zeiss PALM LMD dissection module
- Zeiss AxioCam
- Zeiss Palm Robot acquisition software
Light Source:
- Dissection laser: UV
- Imaging: Excellitas X-Cite 120Q
Filter cubes:
- DAPI
- GFP
- Rhodamine
- Cy5
Location
Helen Diller (MB)
Room
HD 355
Core
LCA
Technique
Brightfield, DIC, phase
Inverted microscope
Live Cell
Microdissection
Widefield epifluorescence
Staff contact
Sarah Elmes
Special Access requirements
Laser Safety
Training by Core Staff