The Hare is a Zeiss microscope equipped with a Yokogawa CSU-X1 and a Photometrics Prime 95B sCMOS for spinning disk confocal imaging and a Hamamatsu Orca-ER sCMOS for widefield imaging. The system is set up for 4-Channel fluorescence and for live cell imaging with temperature and CO2 control.
Capabilities
- High speed, live-cell confocal imaging
- Live-cell dynamics (FRAP, FRET, GCaMP, FURA)
- 4-Channel Fluorescence imaging
Objectives:
- 10X/.25
- 20X
- 40X air
- 40X oil
- 63X oil
Equipment Details:
- PhotometricsPrime 95b sCMOS camera
- Hamamatsu Orca-ER Cmost camera
- Motorized (x, y, z) stage
- Incubator & CO2 Control
- Definite Focus
- DirectFRAP
- Zen Blue Software
Light Source:
- Zen Laser Launch
- Metal halide
- LED illumination
Excitation Wavelengths for Confocal:
- 405nm
- 488nm
- 561nm
- 640nm
Excitation Filters for Widfield:
- DAPI
- GFP
- RFP
- mCherry
- Cy5
Emission Filters:
- DAPI
- GFP
- RFP
- mCherry
- Cy5
Location:
Gladstone Institutes
Room:
Gladstone 327
Core:
GHLMC
Type:
Light
Technique:
Brightfield, DIC, phase
Confocal
FRAP/Photoactivation
High-speed imaging (>100 fps)
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff