The FinWhale is a Zeiss Z.1 Light sheet microscope that can be used to image larger aqueous and cleared samples. The microscope is equipped with dual cameras for 2-Color simultaneous acquisition. The system can be used for high-speed fixed or live-cell imaging, imaging large thick samples at high resolution with low photobleaching and phototoxicity, and isotropic resolution. Samples are typically embedded in agarose and either aqueous or pre-cleared (Clarity, PACT, etc..). Samples can require extensive optimization. Please contact for more information!
Capabilities
- High-speed fixed or live-cell imaging (>100fps)
- Ability to image large, thick samples at high resolution with extremely low-bleach, low phototoxicity
- Long-term live-cell imaging (>6 hours) cabaplities
- Isotropic resolution- best possible reconstruction
- 3D Volumetric Imaging with automated 360 degree sample rotation capabilities
- Tissue Clearing Imaging (acquous and organic solvants)
- Whole tissue, organoid, organism and small organism imaging
Objectives:
- LSFM 5X/0.1
- EC PLAN-NEOFLUAR 5X 0.16
- LSFM10X
- W PLAN-APOCHROMAT (DIC UV-VIS-IR) Clearing 20X/1.0
- W PLAN-APOCHROMAT (DIC VIS-IR) Clearing 40X/1.0
Equipment Details:
- Zeiss Z.1 Lightsheet
- 2 PCO.edge sCMOS camera
- ZEN software
Light Source:
- Zeiss Laser Launch
Excitation Filters/Wavelengths (lamp vs lasers)
- 405nm
- 458nm
- 488nm
- 514nm
- 561nm
- 647nm
Location:
Gladstone Institutes (MB)
Room:
Gladstone Rm. 327
Core:
GHLMC
Technique:
Brightfield, DIC, phase
High-speed imaging (>100 fps)
Lightsheet
Live Cell
Staff contact:
Special Access requirements:
Laser Safety
Training by Core Staff