The Nikon AZ100M, also known as the "Macro Confocal," is a unique addition to the BIDC family. The Macro Confocal is a laser scanning confocal with three low-mag objectives, all with extremely long working distances. Not only does this microscope utilize digital zooming like other laser scanning confocal microscopes, it also boasts a 1-8x optical zoom, making the Macro a one platform solution for whole specimen to single cell imaging.

It uses four laser lines for excitation including 405, 488, 561, and 640 nm. The Macro Confocal has three standard PMTs (Nikon C2-DU3) with a detection range from 400 - 700 nm, as well as a spectral detection (Nikon C2-DUS) with a detection range from 400 - 750 nm. The instrument can achieve up to 100 fps in "fast" mode, and the objectives can cover a field of view over 1 cm.

This microscope can produce confocal as well as diascopic DIC images and uses NIkon Elements software for both standard and spectral imaging. Spectral unmixing is available with this Elements pack, with preset or custom references.

Capabilities

• Live imaging with temperature-controlled incubation
• 1-8x optical zoom
• Fast scanning up to 100 fps
• Up to 1cm field of view

Objectives:

• AZ Plan Apo 1x Air, N.A. 0.1, WD 35mm
• AZ Plan Apo 4x Air, N.A. 0.4, WD 20mm
• AZ Plan Fluor 5x Air, N.A. 0.5, WD 15mm

Excitation Filters/Wavelengths (lamp vs lasers)

• 405nm
• 488nm
• 561nm
• 640nm