The Leica SP5 laser scanning confocal system is used for advanced multi-channel fluorescence and live-cell applications. Excitation sources include five lasers generating  nine lines (405, 458, 476, 488, 496, 514, 543, 594,  633 nm). The inverted microscope system is encased within an incubator for live-cell imaging. An acousto-optical beam splitter allows the investigator to rapidly switch collection parameters without the mechanical movement required of dichroic mirrors. Emission is split into a spectrum using a prism and is collected through five separate tunable photomultiplier tubes. Additionally, a parallel transmitted light image can be collected. 

The system also contains spectral scanning, three-dimensional (z) acquisition and time-lapse capabilities. A programmable stage allows automated return-to-site and multiple-field stitching. 

Capabilities

• Live imaging

Objectives:

• HCX Plan Fluotar Ph1 10x Air, N.A. 0.3, WD 11mm
• N Plan LWD 20x Air, N.A. 0.4, WD 7.4mm
• HC Plan Apochromat 20x Oil, N.A. 0.7, WD 0.26mm
• HCX Plan Apochromat 40x Oil, N.A. 1.25, WD 0.1mm
• HCX Plan Apochromat 63x Oil, N.A. 1.4, WD 0.1mm

Light Source:

• (e.g. Lumencore Spectra X)

Excitation Filters/Wavelengths (lamp vs lasers)

• 405nm
• 458nm
• 476nm
• 488nm
• 496nm
• 514nm
• 543nm
• 594nm
• 633 nm