Zeiss Cell Observer Spinning Disk Confocal

Live cell imaging microscope with full environmental control

This spinning disk confocal microscope features full environmental control and focus control for long time-lapse multichannel live cell and tissue imaging, software controlled motorized stage and objective turret for large area tiling and multi position/well acquisition and z-stacking.

 

Capabilities

  • Time lapse imaging
  • Full environmental control
  • z-stack
  • Multichannel acquisition
  • Multiposition acquisition
  • tiling

 

 


 

Objectives:

  • Plan-Neofluar 2.5x 
  • APlan Apo 10x N.A. 0.45
  • Plan Apo 20x N.A. 0.85
  • Plan-Neo 40x N.A. 0.6
  • Plan Apo 63X Oil N.A. 1.47
  • Alfa Plan Apo 100X Oil N.A. 1.5

 

Equipment Details:

  • Zeiss Axio imager
  • Yokagawa CSU X1 spinning disk module
  • Evolve EMCCD camera on the confocal arm
  • AxioCam 506M on the epifluorescence arm
  • Zeiss ZEN 2012 Acquisition software

Light Source:

  • Excellitas X-Cite 120Q
  • Zeiss Laser Launch

Excitation Wavelengths (Lasers):

  • 405nm
  • 488nm
  • 561nm

Emission Filters:

  • DAPI
  • GFP
  • mCherry
  • Cy5

 

Location
Helen Diller (MB)
Room
HD337
Core
LCA
Technique
Brightfield, DIC, phase
Confocal
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact
Anna Celli
Special Access requirements
Training by Core Staff
Grants/Funding to be acknowledged
NIH P30CA082103