This system is a high-content imaging system providing both spinning disk confocal and widefield imaging. It is optimized for imaging multi-well plates and has both air and water-immersion objectives. It also has temperature and CO2 control for live-cell imaging.
Equipment Details:
- ImageXpress Confocal HT. ai High-Content Imaging System
- Andor Zyla 4.5 camera
- Dual disk unit allowing selection between 3 modes
- 60 micron pinhole disk for your basic confocal requirements
- 50 micron slit for high throughput confocal requirements
- widefield imaging mode.
- Gas Mixer
- MetaXpress Imaging and Analysis software
Objectives:
- Plan Apo Lambda 2x/0.1NA (default overview objective)
- Plan Apo 10x/0.45NA
- Apo LWD 20x/0.95NA WI
- Apo LWD 40x/1.15NA WI
Option: Plan Apo Lambda 4x/0.2NA to be used instead of the 2X
Illumination
- White LED for transmitted light
- Two 89 North LDI laser combiners for a total of 7 laser lines (405, 445, 475, 520, 555, 640 and 730nm)
Filters:
Dichroics:
-
FF409-Di03 (409 nm LP)
-
FF506-Di03 (506nm LP)
-
FF593-Di03-25 (593nm LP)
-
ZT 445/520 (dual BP)
- ZT 405/470/555/640/730 (penta)
Excitation filters
- 445/20 nm
- 467.5/21 nm
- 520/10 nm
- 555/X nm
- 638/17 nm
- 725/30 nm
Emission filters
- 483/32 nm
- 520/28 nm
- 562/40 nm
- 598/25 nm
- 598/25 nm
- 692/40 nm
Location:
Weill Institute for Neurosciences Building (MB)
Room:
472B
Core:
Innovation Core (Weill)
Type:
Light
Technique:
Brightfield, DIC, phase
Confocal
HCA on Multi-well plates
Inverted microscope
Live Cell
Widefield epifluorescence
Staff contact:
Special Access requirements:
Training by Core Staff